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Image Search Results
Journal: Hepatology (Baltimore, Md.)
Article Title: Interleukin-33 facilitates liver regeneration through serotonin-involved gut-liver axis
doi: 10.1002/hep.32744
Figure Lengend Snippet: P70S6K is involved in serotonin mediated hepatocyte proliferation. (A,B) Immunoblotting of phospho-p70S6K/p70S6K was performed in WT and ST2−/− livers at 0 h and 12 h after PHx. The ratios of p-p70S6K/β-actin were quantified (n = 3/group). (C,D) WT and ST2−/− livers treated with saline or DOI were collected at 12 h after PHx. Immunoblotting of phospho-p70S6K/p70S6K was performed and the ratios of p-p70S6K/β-actin quantified (n = 3/group). (E,F) Primary murine hepatocytes were isolated and treated with DOI after serum starvation. Phospho-p70S6K was measured by immunoblotting, and the ratios of p-p70S6K/β-actin from three independent experiments were quantified. (G,H) Primary murine hepatocytes were treated with DOI or an p70S6K inhibitor (p70S6Ki). The levels of PCNA were measured by immunoblotting. The ratios of PCNA/β-actin from three independent experiments were quantified. Two-way ANOVA was performed in (B), (D), (F), and (H)
Article Snippet: After 4 h, the culture medium was deprived of fetal bovine serum (FBS) for at least 48 h. Subsequently, hepatocytes were treated with 5 μM DOI or 10 μM 70
Techniques: Western Blot, Saline, Isolation
Journal: Hepatology (Baltimore, Md.)
Article Title: Interleukin-33 facilitates liver regeneration through serotonin-involved gut-liver axis
doi: 10.1002/hep.32744
Figure Lengend Snippet: Proposed mechanism of IL-33/ST2 regulated liver regeneration. After PHx, IL-33/ST2 signaling in enterochromaffin cells enhances serotonin release. Through the portal blood, serotonin travels into the liver where it binds to HTR2A and activates p70S6K, thereby contributing to hepatocyte proliferation
Article Snippet: After 4 h, the culture medium was deprived of fetal bovine serum (FBS) for at least 48 h. Subsequently, hepatocytes were treated with 5 μM DOI or 10 μM 70
Techniques: